Welcome to the Resume of Sasha Bakhru

Welcome! I am Sasha Bakhru, an engineering science graduate student who has studied biomedical engineering (undergraduate major, BS), mechanical engineering (undergraduate minor, BS), and materials science and engineering (graduate major, MSE) in an effort to gain a fundamental understanding of materials design, fabrication, and characterization for tissue engineering applications. My present work focuses on the design of polymeric hydrogel-based scaffolds for ex vivo neural stem cell expansion.

I have worked towards engineering a microcapsule-based platform for investigating the influence of various biochemical (e.g. FGF2, laminin) and topographical cues on expansion and differentiation. In particular, I have employed a methylated collagen, prepared by esterification of a small fraction of the carboxylic acid groups of glutamic and aspartic acid residues on native porcine collagen (polycationic), and synthetic terpolymer, poly(HEMA:MAA:MMA) (polyanionic) to generate microcapsules by complex coascrevation at the interface of droplets of methylated collagen solutions containing stem cell suspensions and receiving baths of terpolymer solutions.

The methylated collagen imparts substrate functionality through RGD cellular binding sequences, while the relative amounts of the terpolymer constituents - HEMA, MAA, and MMA - can be tuned to control capsule wall charge density and permeability, each of which affects mass transport of nutrients, wastes, gasses, and signaling factors into and out of the microcapsule.

I have encapsulated adult rat neural stem cells in an effort to understand the influence of functionalized 3D microenvironments on this otherwise substrate-adherent, pluripotent cells. The results of this work are in preparation for publication.

Sasha H. Bakhru

Sasha Bakhru
Materials Diagnostics, Inc.
New York, New York 10027
Telephone: (908) 966-3584; Fax: (518) 449-1744

Sasha Bakhru's Sub-sites (Sitemap)
Research - research.sashabakhru.com
Publications - publications.sashabakhru.com
Photos - photos.sashabakhru.com

PUBLICATIONS AVAILABLE ONLINE
L. Eisele, S. Bakhru, X. Liu, R. MacColl, M. Edwards. “Studies on C-phycocyanin from cyanidium caldarium, a eukaryote at the extremes of habitat,” Biochim. Biophys. Acta , 1456, pp.99-107, 2000.
[Article Link]

S. Hong, Z. Tang, D. Djukic, A. Tucay, S. Bakhru, R. Osgood, J. Yardley, A. West, and V. Modi. “Simulation and Experimental Validation of Electroosmotic Flow in a Microfluidic Channel,” Proceedings of the 7th IEEE Conference on MEMS (2001)
[Article Link]

EDUCATION

B.S., School of Engineering, Columbia University (May, 2003)
Major: Biomedical Engineering
Minor: Mechanical Engineering
Senior Thesis Topic: “Polymer Encapsulation of Cells for Controlled Delivery of Drugs”

RESEARCH INTERESTS

Polymer-based drug delivery systems; Micro-total analysis systems, microfluidics and biological microelectromechanical systems (bioMEMS) design and fabrication; microencapsulation of cells for drug delivery applications; characterization of material and mechanical properties of soft tissues, including but not limited to, permeabilities, stress relaxation, and streaming potentials; Bioreactor design and fabrication;

EXPERIENCE

Columbia University Microelectronics Sciences Laboratories
June 2003 – Present: Serve as a part-time, non-matriculated graduate research assistant, under Professor Richard Osgood, Jr., characterizing the effects of elemental doping, by ion beam, on selected ferroelectric materials by transmission electron micrographing and ion beam analysis including Nuclear-Reaction Based Depth Profiling (NRBDP), Rutherford Backscattering Spectrometry (RBS), Particle Induced X-ray Emission (PIXE). Using dopants to achieve Crystal Ion Slicing (CIS) of these materials, highly sensitive sensing and actuating films have been developed for inclusion in MEMS delivery and analytical systems in house at Columbia (see publications).

Sept. 1999 - Jun 2001: Served as an undergraduate research assistant under Professors Richard Osgood, Jr. and James Yardley. Developed piezoelectric actuating systems, microfluidic circuits for bioMEMS applications, and developed a novel technique for surface treatment of various silicones with reactive ionic species via ion implantation through engineered attenuating films (see publications).

Materials Diagnostics, Inc.
May 2003 – Present: Serve as a junior research associate; operate the Dynamitron, Tandem, and Extrion linear particle accelerators. Responsible for Rutherford Backscattering Spectrometry (RBS) and Particle Induced X-ray Emission (PIXE) analyses, as well as ion implantation. (see publications)

Columbia University Orthopaedic Research Laboratories
Jun. 2001 – Dec. 2002: Served as an undergraduate research assistant, independently developing a High Pressure Nano-Perfusion (HPNP) Bioreactor for characterizing strain dependent permeability, streaming potentials, stress relaxation, and drag-induced compaction in both ordinary and engineered soft tissues.

State University of New York at Albany Sept 2000- PRESENT: Serve as a part-time machinist.

Hewlett Packard, Inc.
Jun. 1999 - Aug. 1999: Designed and built a micro-electromechanical, liquid chromatography system under the guidance of Dr. Alain Kaloyeros. The device is now in use at the biochemistry core of the Wadsworth Research Laboratories of the New York State Department of Health.

New York State Center for Advanced Thin Film Technologies
Sept. 1998 – Jun 1999: Played an integral role in the development of a chem-optical thin-film gas sensing system for detecting small quantities of ambient carbon monoxide. Specifically, designed and built a miniature, confocal system of lenses, which introduced monochromatic light into the system for spectroscopic measurement, along with the optical stages upon which the thin films were deposited.

New York State Department of Health’s Wadsworth Laboratories Jun. 1998 – Sept. 1998: Modeled protein superstructure and characterized protein denaturation in thermophilic, light-harvesting bacteria from the hot springs of Yellowstone National Park. The work was sponsored by, and conducted under the supervision of Dr. Robert MacColl. (see publications)

SKILLS

Adept as a machinist; Have logged over 250 hours in clean fabrication environments; Familiar with Matlab 6.0, Mathematica, Alias Wavefront, Java, and C; Proficient in sterile culture technique; Proficient at mold-making; Proficient at glass blowing; Fluent in Spanish.

TA POSITIONS HELD

E1001 Engineering in Medicine (Fall 2000, Fall 2001, Fall 2002)
E4502 Tissue Engineering II (Spring 2002)

SOCIETAL MEMBERSHIPS AND POSITIONS HELD

IEEE Engineering in Medicine and Biology Society
President, Columbia University Student Branch Chapter (2000)
    “Starting Your Own University Branch Chapter”
    By: Sasha Bakhru, published in the EMBS Magazine (2001)

Biomedical Engineering Society
President, Columbia University Student Branch Chapter (2001-2002)

AWARDS AND FELLOWSHIPS WON

Fellowships:

National Science Foundation Materials Research in Science and Engineering Summer Research Fellowship, Columbia University (Summer 2000)
Research Foundation, State of New York, Summer Research Fellowship (Summer 1999) (Awarded for study at the New York State Center for Advanced Thin Film Technology in Albany, New York)

Awards:

Outstanding Service Award, Teaching Assistant, E1001 Engineering in Medicine, Columbia University (Fall Semester, 2002)
Outstanding Service Award, Teaching Assistant, E1001 Engineering in Medicine, Columbia University (Fall Semester, 2001)
Gold Crown Award for Outstanding Service to the School Community (Columbia University, 2001)

PUBLICATIONS

Journal Articles

T. Izuhara, R. Roth, R. Osgood, S. Bakhru, H. Bakhru. "Low-voltage tunable TE/TM converter on an ion-sliced lithium niobate thin film," Electron. Lett. (2003) Accepted

R. Vanga, M. Levy, Y. Hong, K. Moon, S. Bakhru, and H. Bakhru. “Single-Crystal Relaxor Ferroelectric Piezoactuators with Interdigitated Electrodes,” Ferroelec. Lett. In Press

K. Moon, M. Levy, Y. Hong, S. Bakhru, and H. Bakhru. “Microscale Patterning of Mesoscopic PZN-PT Single Crystal Films by Crystal Ion Slicing and Laser-Induced Etching,” Ferroelec. Lett. 30(1-2), 2003.

M. Levy, S. Ghimire, A. Bandyopadhyay, Y. Hong, K. Moon, S. Bakhru, and H. Bakhru. "PZN-PT Single-Crystal Thin Film Monomorph Actuator, " Ferroelec. Lett., 29(3-4), pp.29-40, 2002.

S. Bakhru, H. Bakhru, “Selective Fluorination of Polymer Surfaces at the Sub-Micron Scale,” J. Ion-Beam Tech. 12(2-3), 2001.

L. Eisele, S. Bakhru, X. Liu, R. MacColl, M. Edwards. “Studies on C-phycocyanin from cyanidium caldarium, a eukaryote at the extremes of habitat,” Biochim. Biophys. Acta , 1456, pp.99-107, 2000.

Conference Papers

S. Bakhru, H. Bakhru. “Fluorine Migration in Ion-Beam Doped Biopolymers,” Presented at the IEEE 2003 Meeting

T. Izuhara, R. Roth, R. Osgood, S. Bakhru, and H. Bakhru. "A Low-voltage Tunable Electro-optical Polarization Converter on Freestanding Thin-Film LiNbO3," Technical Digest. Postdeadline session. CLEO '03. Conference on Lasers and Electro-Optics, CThPDB5 (2003)

S. Hong, Z. Tang, D. Djukic, A. Tucay, S. Bakhru, R. Osgood, J. Yardley, A. West, and V. Modi. “Simulation and Experimental Validation of Electroosmotic Flow in a Microfluidic Channel,” Proceedings of the 7th IEEE Conference on MEMS (2001)

S. Bakhru, R. Osgood, and J. Yardley. “The Fabrication and Evaluation of Novel Microfluidic Systems in Polymers," NSF Undergraduate Summer Fellows Conference, Columbia University (2000)